Molecular cloning, expression and IgE-immunoreactivity of phospholipase A1, a major allergen from Polybia paulista (Hymenoptera: Vespidae) venom
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چکیده
منابع مشابه
Hyaluronidase from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): Cloning, structural modeling, purification, and immunological analysis.
In this study, we describe the cDNA cloning, sequencing, and 3-D structure of the allergen hyaluronidase from Polybia paulista venom (Pp-Hyal). Using a proteomic approach, the native form of Pp-Hyal was purified to homogeneity and used to produce a Pp-specific polyclonal antibody. The results revealed that Pp-Hyal can be classified as a glycosyl hydrolase and that the full-length Pp-Hyal cDNA (...
متن کاملHeterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom
Polybia paulista (Hymenoptera: Vespidae) is responsible for a high number of sting accidents and anaphylaxis events in Southeast Brazil, Argentina and Paraguay. The specific detection of allergy to the venom of this wasp is often hampered by the lack of recombinant allergens currently available for molecular diagnosis. Antigen 5 (~23 kDa) from P. paulista venom (Poly p 5) is a highly abundant a...
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93 Purification of the Allergenic Protein Hyaluronidase From the Venom of SocialWasp Polybia Paulista (Hymenoptera: Vespidae) Débora Laís Justo Jacomini, MD, Franco Dani Campos Pereira, 1 José Roberto Aparecido dos Santos Pinto, MD, Lucilene Delazari dos Santos, PhD, Anally Ribeiro da Silva Menegasso, Mário Sérgio Palma, PhD, Ricardo de Lima Zollner, PhD, and Márcia Regina BrochettoBraga, PhD. ...
متن کاملMolecular Cloning, Characterization, and Expression of Cuc m 2, a Major Allergen in Cucumis melo
Background: Several studies reported the clinical features of IgE-mediated hypersensitivity after ingestion of melon. Melon allergy is a common IgE-mediated fruit allergy in Iran. This prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the IgE-binding capacity of the natural protein with the recombinant allergen, and to determine ...
متن کاملMolecular Cloning and Expression of Cro s 1: an Occupational Allergen from Saffron Pollen (Crocus sativus)
Background: The cultivation of saffron is expanding through the southeast of Iran, and allergy to saffron pollen occurs in workers involved in processing this plant. We aimed to clone, sequence and express a major allergen involved in saffron pollen allergy, and to compare the recombinant with the natural allergen. Methods: The N-terminal amino acid sequence of Cro s 1, an allergen from saff...
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ژورنال
عنوان ژورنال: Toxicon
سال: 2016
ISSN: 0041-0101
DOI: 10.1016/j.toxicon.2016.11.006